Isolation, purification and characterization of vindoline from Catharanthus roseus

Raghu Nataraj 1, *, Jagadish MS 1, Kavitha Raj Varadaraju 2 and Bindu J 3

1 Division of Molecular Biology, School of Life Sciences, JSS AHER, Mysuru- 570015, Karnataka, India.
2 DST-WOSA, School of Life Sciences, JSS AHER, Mysuru-570015, Karnataka, India.
3 ICMR-SRF, Division of Molecular Biology, School of Life Sciences, JSS AHER, Mysuru- 570015, Karnataka, India.
 
Research Article
International Journal of Science and Research Archive, 2023, 08(01), 310-319.
Article DOI: 10.30574/ijsra.2023.8.1.0047
Publication history: 
Received on 02 December 2022; revised on 15 January 2023; accepted on 18 January 2023
 
Abstract: 
Catharanthus roseus is a popular medicinal plant available all over the world. Across the globe, different parts of the plant are used to treat diabetes, hypertension, cancer, and menstrual abnormalities. In the current study, aim was to isolate, purify and characterize vindoline from the aerial parts (stem, leaves and flowers) of C.roseus. Vindoline was extracted by hot ethanolic extraction procedure and then purified using preparative thin layer chromatography (pTLC). The functional group analysis was performed to authenticate the presence of specific functional groups by using fourier transform- infrared spectroscopy (FT-IR). Liquid chromatography-mass spectroscopy (LC-MS) and high performance liquid chromatography (HPLC) were performed to conform the purity of the isolated compound and the scientific data presented.
 
Keywords: 
Catharanthus roseus ; Vindoline; Hot ethanolic extraction; Preparative TLC; HPLC; LCMS; FT-IR
 
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