Phytochemical screening study in different parts of Chromolaena odorata by LC MS method and related parameters

G. Bhavani Devi 1, K.S. Ramya 2, Dappula Sandhya Sri 1, P. Josthna 3 and C.V. Naidu 1, *

1 Department of Biotechnology, Dravidian University, Kuppam-517426, A.P., India.
2 Department of Biotechnology, Shri Gnanambica Degree College, Madanapalli-517325, A.P. India.
3 Department of Biotechnology, Sri Padmavathi Mahila Visvavidyalayam (Women’s University), Tirupati- 517502, A.P. India.
 
Research Article
International Journal of Science and Research Archive, 2022, 07(02), 128–140.
Article DOI: 10.30574/ijsra.2022.7.2.0239
Publication history: 
Received on 01 October 2022; revised on 05 November 2022; accepted on 08 November 2022
 
Abstract: 
Chromolaena odorata (Syn: Eupatorium odoratum L.) (Asteraceae) is a perennial herb consisting of biologically potent chemical. It is mainly found in the humid tropics and sub-tropics in worldwide. C. odorata displayed allelopathic effects and have been reported to cause livestock death. C. odorata is used against dysentery, malaria, diarrhea, wound healing, toothache and headache in traditional medicine. In the present study, we investigated the assays tested for the presence of several phytochemicals (alkaloids, terpenoids, flavonoids, saponins, phenols, cardiac glycosides, resins and anthraquinones) in different extracts which included ethanol, methanol, petroleum ether and aqueous. All the extracts were tested against Bacteria. Furthermore, we studied phytochemical screening by QTOF-MS analysis it represents various acids, flavonoids etc. Phytochemical profiling revealed the presence of approximately 20 compounds each from the leaf and stem extracts whereas root consisted of 10 compounds. Among these, the main compounds were Vanillic acid, Chlorogenic acid, Benzoic acid, Genkwanin, Naringenin and Luteolin. The ethanol and methanol extracts were found active alongside the tested bacteria as they showed potential phytochemical constituents. The bacteria, Escherichia coli and Bacillus subtilis showed promising activity against ethanol and methanol leaf, stem and root extracts which proved that the plant extracts are potential candidates for antibiotic resistance against such bacteria. In addition, the Chlorogenic acid present in leaf, stem and root extracts of the plant helps process blood sugar in a way that helps prevent and reduce the number of blood sugar spikes. It also helps boost metabolism, helping people with diabetes maintain a healthy weight.
 
Keywords: 
QTOF-MS; Chromolaena odorata; Acids and phenolic compounds Phytochemical screen­ing; Various extracts (Ethanol; Methanol; Petroleum ether and Aqueous); Antibacterial activity
 
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