Isolation, characterization and identification of root nodule bacteria from Macrotyloma uniflorum (Lam.) Verdc

Anita Tudu 1, *, Ghazala Shaheen 2, Vinay Oraon 3 and Latika Sharan 2

1 Department of Botany, Ranchi Women’s College, Ranchi University, Ranchi, Jharkhand, India.
2 Department of Botany, Ranchi University, Ranchi, Jharkhand, India.
3 Department of Biotechnology, University Department of Botany, Ranchi University, Ranchi, Jharkhand, India.
 
Research Article
International Journal of Science and Research Archive, 2024, 12(01), 417–425.
Article DOI: 10.30574/ijsra.2024.12.1.0780
Publication history: 
Received on 21 March 2024; revised on 04 May 2024; accepted on 07 May 2024
 
Abstract: 
The present study was conducted to characterize the native plant growth promoting rhizobacteria (PGPRs) from the nodules of Macrotyloma uniflorum (Lam.) Verdc. The seeds of Macrotyloma uniflorum was grown in two different soils i.e., sterile and non-sterile soil and four bacterial isolates were isolated. The bacterial isolates were characterised on the basis of morphological and different biochemical analysis. Phylogenetic identification was done on the basis of 16S rRNA analysis. Four isolates obtained belongs to Bacillus velezensis and Enterobacter cloacae.
 
Keywords: 
PGPRs; Root nodules; Rhizobacteria; Macrotyloma uniflorum; 16S rRNA
 
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