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Effect of ethanol and aqueous extract of Moringa oleifera on bacteria isolated from wound infection
Department of Biology, College of Sciences, Mustansiryah University, Baghdad, Iraq.
Research Article
International Journal of Science and Research Archive, 2023, 09(02), 941–949.
Article DOI: 10.30574/ijsra.2023.9.2.0642
Publication history:
Received on 28 June 2023; revised on 22 August 2023; accepted on 25 August 2023
Abstract:
The aqueous and ethanol extracts of Moringa oleifera used for the treatment of infectious disease were tested for their active against gram positive and gram-negative bacteria isolated from burns infection culture using the broth dilution and disc diffusion method. Results of this study revealed the presence of phytochemical which were active against gram positive and negative bacteria. Ethanol extracts of plant showed highest activity other than aqueous extract. The minimum inhibitory concentration (MIC) of the aqueous extracts on the test organism was 25- 100 mg\ml, while that of the ethanol extract ranged between 25 -50 mg\ml on the test organisms, the minimum bacterial concentration (MBC) ranging between 25-100 mg\ml for ethanol extract, and 25-200 mg\ml for aqueous extracts. The highest activity at 45 oC was demonstrated by the ethanol extracts of S. rosmarinus against Staphylococcus aureus and Klebsiella spp. In this study plant extracts against gram negative bacteria showed activity in acidic pH only in contrast of gram positive bacteria which were constant in plant extract. M. oleifera contained essential elements at higher levels. The results of this study suggest the possibility of using the ethanolic extracts of plant in treating diseases caused by the test organisms, especially when prepared at acidic pH.
Keywords:
Plant extract; Moringa oleifera; Antimicrobial activity; Bacteria
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Copyright © 2023 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
