Antioxidant and immunological evaluation of Ceratonia siliqua on Methotrexate induced Albino mice male

Carob (Ceratonia siliqua) plant was frequently used to cure a variety of health issues. This study aimed to evaluate the total flavonoid, phytochemical compounds, antioxidant activity In vitro, as well as immunological potential of Ceratonia siliqua methanolic extract in vivo through determination of total and absolute count of white blood cell on albino male mice. The result showed that Ceratonia siliqua contain (224.5±37.86) of flavonoid compound, in addition of many bioactive phytochemical compounds like (tannin, glycoside, alkaloid, polyphenol, and glycoside). Antioxidant activity indicated that the ability of methanolic extract of Ceratonia siliqua plant to scavenging free radical In vitro in all concentration uses (0.04, 0.08, 0.16, 0.32 and 0.64 mg/ml) when compared with trolox (vit E). Immunological parameters indicated the ability of plant to enhance the immunity by increase WBCs count (7166 cell/cu.mm.blood) in comparison with positive (methotrexate) and negative control, as well as increase in lymphocytes (3700 cell/cu.mm.blood) , neutrophil (2985 cell/cu.mm.blood) and monocyte (481 cell/cu.mm.blood) All of these effect attributed to active phytochemical constituents in plant extract.