Study and analysis of the structural-biochemical characteristics in Mollicutes biofilms formation

Villegas-Reyes G 1, Rivera A 2, *, Giono-Cerezo S 3, Cedillo-Ramírez ML 2 and Vega-Benítez M 4

1 Faculty of Biology, Benemérita Universidad Autónoma de Puebla, México.
2 Laboratory of Mycoplasmas, Benemérita Universidad Autónoma de Puebla, México.
3 National School of Biological Sciences, Instituto Politécnico Nacional, CDMX.
4 Central Regional Complex, Amozoc-Puebla, Benemérita Universidad Autónoma de Puebla, México.
 
Research Article
International Journal of Science and Research Archive, 2024, 11(01), 057–062.
Article DOI: 10.30574/ijsra.2024.11.1.0005
Publication history: 
Received on 26 November 2023; revised on 04 January 2024; accepted on 07 January 2024
 
Abstract: 
Biofilms have a wide distribution in various environments and even in devices used in medical practice, for this reason its relevance to study these structures and consider strategies to prevent their development and the effects they imply. Due to the medical importance that mycoplasmas represent, the objective of the present work was to analyze the capacity of Mycoplasma fermentans P140 to form a biofilm on an inert surface and to document the characteristics of the process in the formation of biofilm in Mollicutes. The interaction of the culture of Mycoplasma fermentans P140 with intrauterine devices for 72 hours showed by macroscopic analysis the crystal violet staining of the technique to evaluate biofilm formation, stereoscopic microscopy showed the formation of cell aggregates and by scanning electron microscopy the characteristic formation of biofilm. The search for reports referring to studies of biofilm formation in Mollicutes showed that there is recent evidence of biofilm formation in this group of microorganisms, both of veterinary medical interest and of interest in public health. Through metabolomics studies, it will be possible to demonstrate the metabolic products during the formation of biofilms in the different genera and species of these bacteria. Representing these investigations relevance to the field of public health and other ecosystems where health-disease processes are related.
 
Keywords: 
Mollicutes; Biofilms; Characteristics; Dynamic; Health-disease processes
 
Full text article in PDF: