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Protocol for dissection of Drosophila abdomens for fluorescent imaging
1 Osmania Medical College, Hyderabad, Telangana, 500095, India.
2 Department of Biotechnology, Malla Reddy Engineering College, Medchal, Telangana, 500100, India.
Research Article
International Journal of Science and Research Archive, 2024, 12(02), 1211–1216.
Article DOI: 10.30574/ijsra.2024.12.2.1315
Publication history:
Received on 10 June 2024; revised on 24 July 2024; accepted on 26 July 2024
Abstract:
Fluorescent imaging in Drosophila species is indispensable for investigating dynamic biological processes and visualizing gene expression patterns with cellular precision. This technique leverages the transparency of Drosophila larvae and pupae, combined with advanced microscopy, to enable real-time observation of developmental events such as morphogenesis and organogenesis. Genetically encoded fluorescent proteins and dyes allow specific labeling of cells and proteins, facilitating detailed studies of spatial and temporal dynamics within intact tissues. Techniques like confocal and two-photon microscopy provide high resolution and depth penetration, essential for 3D reconstruction and quantitative analysis of complex biological structures. Fluorescent imaging in Drosophila supports disease modeling, drug screening, and therapeutic exploration, bridging insights from basic biology to potential clinical applications. It is therefore necessary to develop imaging techniques and protocols that accurately capture and profile gene expression patterns in a wide range of Drosophila tissues. In this study, we present a detailed protocol for preparing and imaging transgenic Drosophila abdomen, which will enable researchers investigate gene expression patterns underlying fundamental biological processes in the abdomen.
Keywords:
Drosophila; Imaging; Fluorescent reporters; Abdomen
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Copyright © 2024 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
