Molecular identification of bacteria (Klebsiella aerogenes, PP335235) isolated from street food (Fried Rice); study of its antibiotic sensitivity pattern --------- with Lactobacillus gasseri: A nutraceutical remedial approach

Parivendhan M 1, *, Harini M 1, Dharaniya A 1, Kansiya N 1, Ragunathan R 2 and Saravanan N 1

1 Department of Biotechnology, Muthayammal Engineering College, Rasipuram, Tamil Nadu, India.
2 Department of Biotechnology, Centre for Bioscience and Nanoscience Research, Eachanari, Coimbatore, India.
 
Research Article
International Journal of Science and Research Archive, 2024, 11(02), 1198–1216.
Article DOI: 10.30574/ijsra.2024.11.2.0517
 
Publication history: 
Received on 18 February 2024; revised on 01 April 2024; accepted on 04 April 2024
 
Abstract: 
Street food is an integral part of India's culinary culture, offering patrons a wide range of affordable options. However, concerns about the public's health risks associated with street food consumption persist due to inadequate infrastructure and the transient nature of street vendors. Contaminated street food can lead to foodborne illnesses such as gastroenteritis, typhoid fever, pneumonia, food poisoning, and hepatitis A. Probiotics, which are beneficial bacteria, are potentially effective by targeting specific infections. Probiotic-enriched fruit drinks present an innovative approach to improving population nutrition and probiotic provision. Lactobacillus gasseri is a gram-positive bacterium that has been demonstrated to be a promising strain of probiotic due to its resistance to acidity and beneficial effects on gut health. Alginate encapsulation improves the stability and release of probiotic strain. Probiotic fruit juices can be made from fruits that are rich in antioxidants and nutrients, such as Passiflora ligularis, Fragaria ananassa, and Phyllanthus emblica. The aim of this study was to use molecular methods to discover Klebsiella aerogenes (PP335235) isolated from street foods. The study also sought to determine the isolate's pattern of antibiotic sensitivity and explore the potential of fortifying fruit juice with Lactobacillus gasseri as a probiotic nutraceutical therapeutic strategy.
 
Keywords: 
Street food pathogens; Probiotics; Lactobacillus gasseri; Klebsiella aerogenes; Alginate encapsulation
 
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