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Method development and validation of nifedipine and lignocaine by RP-HPLC
Department of Pharmaceutical Analysis, Srikrupa Institute of Pharmaceutical Sciences, Siddipet, Telangana, India-502277.
Research Article
International Journal of Science and Research Archive, 2023, 08(01), 565–569.
Article DOI: 10.30574/ijsra.2023.8.1.0115
Publication history:
Received on 21 December 2023; revised on 31 January 2022; accepted on 02 February 2023
Abstract:
For the validation of the nifedipine and lignocaine assay by reverse phase high performance liquid chromatography, in both pure form and tablet dosage form, a straightforward, quick, and exact approach has been established. ACN, Methanol, and perchloric acid were used as the mobile phase in chromatography on a Kromasil 100-5-C18 (4.6 x 150 mm, 5 m) column at a flow rate of 1.0 ml/min. 210 nm was used for detection. Nifedipine and lignocaine had retention times of 3.30 and 5.820.02 min, respectively. In the concentration range of 10–50 mg/ml of nifedipine and 20–100 mg/ml of lignocaine, the approach yields linear responses. The method precision for the assay result was less than 2.0%RSD, and the Nifedipine and lignocaine individual assays should be between 98% and 102.0%, respectively. The technique is helpful for pharmaceutical and bulk formulation quality control.
Keywords:
Nifedipine; Lignocaine; Assay; RP-HPLC; Validation
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Copyright © 2023 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
