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Genetic detection of prolactin intron 1 region in breast cancer patients of Iraqi women
1 Department of biotechnology, College of Science, Baghdad, Iraq.
2 Department, molecular and medical biotechnology, College of Biotechnology City, Baghdad, Iraq.
3 Department of biotechnology, College of Science, Baghdad, Iraq.
4 National Center of Hematology, Mustansiriyah University, Baghdad, Iraq.
Research Article
International Journal of Science and Research Archive, 2024, 12(02), 2327–2340
Article DOI: 10.30574/ijsra.2024.12.2.1482
Publication history:
Received on 03 July 2024; revised on 16 August 2024; accepted on 19 August 2024
Abstract:
This study was made to inspect the genetic cause of breast cancer through the molecular base related with single nucleotide polymorphism (SNP ) at prolactin gene in patients with the breast cancer .This study comprised thirty blood samples from patients suffering from breast cancer. Also thirty tissue samples of breast cancer patients were collected in which samples were formalin fixed paraffin embedded tissue. thirty blood samples from healthy persons were collected served as control group. The main ages of patients were 19 to 60 and same for control (healthy) group.The variation in gene that accountable for synthesis of hormone prolactin, was conducting using samples of breast cancer patients to demonstrate if this variation is important to breast cancer risk In addition to. Polymerase chain reaction (PCR ), was done by using specific set of primers , in which 3 primers were selected to amplify the intron 1 region of the gene.
After optimization of the amplification condition, the product was sent for DNA sequencing for detection of variation of patient prolactin gene, so the association of this variation of prolactin gene to breast cancer patients was clear after studying the intron region. In intron 1 of the gene, 7 mutation was detected by using primer 1, in which two of them is deletion mutation and 5 was substitution, while in same intron of the gene but using primer 2, 6 mutation was detected all is substitution. The risk association between the prolactin intron gene association and breast cancer patients using information on national center for biotechnology information (NCBI), and Mega 6 program.The results of mutation detection in the PRL gene intron region showed that there is occurrence of mutations in of breast cancer patients samples
Keywords:
Single nucleotide polymorphism; Breast cancer; Prolactin intron 1 gene
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Copyright © 2024 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
