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Evaluation of anti-cancer activities of Carcino SC Mammae 200ch in breast cancer cell lines (MDA-MB-231)
Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, Maharashtra, India.
Research Article
International Journal of Science and Research Archive, 2023, 08(01), 592–599.
Article DOI: 10.30574/ijsra.2023.8.1.0116
Publication history:
Received on 19 December 2022; revised on 28 January 2023; accepted on 30 January 2023
Abstract:
Aim/Background: Breast Carcinoma is one of the most common cancers occurring mostly in adolescents and has a very high risk of malignancy. Despite of some challenges with the present therapeutic approaches, alternatives have gained popularity, such as ayurveda, homeopathy, siddha, unani, etc including the use of phytochemicals and various other anticancer compositions. Carcino SC Mammae 200CH is a homeopathic medicinal mother tincture which is not as much therapeutically known for its exceptional health benefits and have to be explored for its effective activities during breast cancer. The current study aimed at evaluating the anti-cancer properties of Carcino SC Mammae 200CH on MDA-MB-231 cell line for human breast cancer, by investigating its apoptotic activity through changes in cell viability.
Materials and Methods: Carcino SC Mammae 200CHwas achieved through local market in Aurangabad, Maharashtra. The MDA-MB-231 cells were treated with ity in the concentration range 3.125 µg/mL to 75µg/mL of the Carcino SC Mammae 200CH and incubated for 24, 48, and 72 hr. The percentage cell viability and IC50 values were obtained. The nuclear changes were examined. GraphPad Prism was used for statistical analysis with significant p-value at <0.05.
Results: The IC50 values obtained for Carcino SC Mammae 200CH were 75µg/mL, 25µg/mL, and 6.25µg/mL for 24, 48, and 72 hr respectively. Change in the nuclear morphology was observed following incubation with Carcino SC Mammae 200CH.
Conclusion: Our result suggests that Carcino SC Mammae 200CH have shown significant effects on MDA-MB-231 breast cancer cells and can be considered to supplement conventional therapeutic strategies.
Keywords:
Apoptosis; Breast Cancer; Carcino SC Mammae 200CH; MDA-MB-231; DAPI staining; MTT assay
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