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Disinfection procedure for stem cuttings and in vitro production of axillary buds for the Persian lime sanitation
1 National Institute for Research on Agriculture, Forestry and Livestock (INIFAP). Ixtacuaco Experimental Field, Km 4.5. Martínez de la Torre-Tlapacoyan Street, Cong. Rojo Gómez, Tlapacoyan, Veracruz, Mexico.
2 Technological University of the Huasteca Hidalguense. Huejutla - Chalahuiyapa highway S/N, Tepoxteco, Huejutla de Reyes, Hidalgo, Mexico.
Research Article
International Journal of Science and Research Archive, 2022, 07(01), 470–476.
Article DOI: 10.30574/ijsra.2022.7.1.0223
Publication history:
Received on 20 September 2022; revised on 25 October 2022; accepted on 28 October 2022
Abstract:
The research work covered the in vitro establishment of Persian lime nodal segments using a twig disinfection protocol, and their culture on Murashige and Skoog (MS) culture media supplemented with antibiotics (Estrepto-Ler® and Rifampicin) and fungicide (Chlorothalonil). The axillary buds production was performed cultured the nodal segments without contamination signs on MS culture media enriched with two cytokinins (6-BAP and Kinetin) at different concentrations. Results showed that 52 % of the nodal segments did not show signs of in vitro contamination when the twig submerged in 20% chlorine for 15 min during disinfection process and nodal segments were grown in MS culture medium supplemented with 2 mg L-1 of Rifampicin and fungicide Chlorothalonil. Interestingly, MS culture medium enriched with 6-BAP (0.5 mg L-1) + Kinetin (0.5 mg L-1) turned out to be the best combination to achieve that 78 % of nodal segments promoted 1 to 2 axillary buds.
Keywords:
Citrus latifolia; In vitro; Phytohormones; Axillary buds
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Copyright © 2022 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
