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Analysis of Cassava (Manihot esculenta Crantz) DNA Patterns and Peroxidase Enzyme Activity Results of Induced Resistance with Salicylic Acid
1 Applied Biology Study Program, Faculty of Mathematics and Natural Sciences, University of Lampung, Bandar Lampung, Lampung, Indonesia.
2 Biology Study Program, Faculty of Mathematics and Natural Sciences, University of Lampung, Bandar Lampung, Lampung, Indonesia.
Research Article
International Journal of Science and Research Archive, 2024, 12(01), 2193–2198.
Article DOI: 10.30574/ijsra.2024.12.1.0872
Publication history:
Received on 20 April 2024 revised on 28 May 2024; accepted on 30 May 2024
Abstract:
Cassava (Manihot esculenta Crantz) is a food commodity that is ranked third after rice and corn, all three are the main sources of carbohydrates and in the future this commodity will have an increasingly strategic role in people's lives and the country's economy. The growth of cassava plants cannot be separated from diseases caused by pathogens, one of which is a fungus which, if it infects the plant, will multiply and spread so that the plant will be damaged. Biological disease control can be done by using superior varieties, one of which is by administering salicylic acid. The aim of this research was to analyze DNA patterns and determine the peroxidase enzyme activity of cassava plants exposed to salicylic acid compared to controls. This research used a Completely Randomized Design (CRD) with one factor, namely the concentration of salicylic acid which was divided into 5 levels, namely 0 ppm, 80 ppm, 100 ppm, 120 ppm, and 140 ppm with 5 replications each. This research data is presented in comparative descriptive form supported by photos and quantitative data tabulated with different concentrations. The research results showed (1) There was a new (specific) DNA band with a size of 1,100 bp (OPB_14), (2) At a salicylic acid concentration of 100 ppm, the highest peroxidase enzyme activity was 0.193 U/mg/minute.
Keywords:
Cassava; Salicylic Acid; Induce Resistance; DNA Patterns; Peroxidase Enzyme Activity
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Copyright © 2024 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
