Acceleration of micropropagation procedure of Bambusa nutans: A commercially important bamboo species

The present communication described an accelerated, efficient and reproducible in vitro propagation procedure for large-scale multiplication of Bambusa nutans Roxb. The nodal segments (12–18 mm) collected from secondary branches of superior clump in the month of April reported enhanced multiple shoot formation (7-8) when implanted on Murashige & Skoog semi solid medium supplemented with 10 µM BA (6-Benzylaminopurine). These axillary shoot cultures pass through ten subculture cycle on liquid medium of 10 µM BA. The cluster of two axillary shoots transferred to liquid MS medium containing 10 µM BA+ 7.0 µM NAA (a-Naphthaleneacetic acid) produced a maximum of 14.5 multiple shoots after 20 days of inoculation. Rooting of 100 % of shoots was achieved in excised propagules when transferred to ½ MS medium supplemented with 30 µM NAA. A two-step hardening procedure of in vitro raised plants ensured 100 % survival. Macroproliferation was carried out in hardened plants after 4 months registered threefold number of plants in polythene bags. The field transferred plants were reported with 100 % survival with prolific new shoot formation and growth.