Genetic detection of prolactin intron 1 region in breast cancer patients of Iraqi women

This study was made to inspect the genetic cause of breast cancer through the molecular base related with single nucleotide polymorphism (SNP) at prolactin gene in patients with the breast cancer .This study comprised thirty blood samples from patients suffering from breast cancer. Also thirty tissue samples of breast cancer patients were collected in which samples were formalin fixed paraffin embedded tissue. thirty blood samples from healthy persons were collected served as control group. The main ages of patients were 19 to 60 and same for control (healthy) group.The variation in gene that accountable for synthesis of hormone prolactin, was conducting using samples of breast cancer patients to demonstrate if this variation is important to breast cancer risk In addition to. Polymerase chain reaction (PCR), was done by using specific set of primers , in which 3 primers were selected to amplify the intron 1 region of the gene.
After optimization of the amplification condition, the product was sent for DNA sequencing for detection of variation of patient prolactin gene, so the association of this variation of prolactin gene to breast cancer patients was clear after studying the intron region. In intron 1 of the gene, 7 mutation was detected by using primer 1, in which two of them is deletion mutation and 5 was substitution, while in same intron of the gene but using primer 2, 6 mutation was detected all is substitution. The risk association between the prolactin intron gene association and breast cancer patients using information on national center for biotechnology information (NCBI), and Mega 6 program.The results of mutation detection in the PRL gene intron region showed that there is occurrence of mutations in of breast cancer patients samples